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The research investigates the impact of urbanization on biodiversity, focusing on critical ecological habitats within Ahmedabad. Rapid urban growth has led to significant habitat degradation, biodiversity loss, and wetland shrinkage, affecting species survival, especially migratory birds. Through detailed analysis of areas like Chandola Lake, Thol Bird Sanctuary, and Vastrapur Lake, the study identifies land-use changes, vegetation loss, and ecological disruptions. It proposes strategic recommendations including ecologically sensitive zoning, green infrastructure mandates, agricultural regulation, and environmental assessments. These measures aim to mitigate urban pressures, preserve biodiversity, and enhance ecosystem resilience amid ongoing urban expansion.